Erin O'Shea, Harvard University: "Reverse Engineering of a Transcriptional Network Regulated by the MAPK Hog1"
Feb 26 2008, 11:00 am
Distinguished Lecture Series Guest Speaker:
Professor of Molecular and Cellular Biology
Investigator, Howard Hughes Medical Institute
Director, FAS Center for Systems Biology
Date & Time:Tuesday February 26, 2008
Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks we developed a new analytical approach, where the data from single and multiple mutant strains are analyzed together, to probe the influence and interaction of network components . We use this strategy to build an accurate, quantitative, and comprehensive model of the Hog1 MAPK dependent osmotic stress response in budding yeast and describe how the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context dependent response. This study lays out a path to identifying, and characterizing the role of, signal integration and processing in other gene regulatory networks.