Prediction of substrate specificity and preliminary kinetic characterization of the hypothetical protein PVX_123945 from Plasmodium vivax.

TitlePrediction of substrate specificity and preliminary kinetic characterization of the hypothetical protein PVX_123945 from Plasmodium vivax.
Publication TypeJournal Article
Year of Publication2015
AuthorsSrinivasan, B, Kempaiah Nagappa L, Shukla A, Balaram H
JournalExperimental parasitology
Volume151-152
Pagination56-63
Date Published2015 Apr-May
Abstract

Members of the haloacid dehalogenase (HAD) superfamily are emerging as an important group of enzymes by virtue of their role in diverse chemical reactions. In different Plasmodium species their number varies from 16 to 21. One of the HAD superfamily members, PVX_123945, a hypothetical protein from Plasmodium vivax, was selected for examining its substrate specificity. Based on distant homology searches and structure comparisons, it was predicted to be a phosphatase. Thirty-eight metabolites were screened to identify potential substrates. Further, to validate the prediction, biochemical and kinetic studies were carried out that showed that the protein was a monomer with high catalytic efficiency for β-glycerophosphate followed by pyridoxal 5'-phosphate. The enzyme also exhibited moderate catalytic efficiencies for α-glycerophosphate, xanthosine 5'-monophosphate and adenosine 5'-monophosphate. It also hydrolyzed the artificial substrate p-nitrophenyl phosphate (pNPP). Mg(2+) was the most preferred divalent cation and phosphate inhibited the enzyme activity. The study is the first attempt at understanding the substrate specificity of a hypothetical protein belonging to HAD superfamily from the malarial parasite P. vivax.

PDFhttp://cssb.biology.gatech.edu/skolnick/img/pdfs/Hypothetical_protein.pdf
10.1016/j.exppara.2015.01.013
Pub Med Link

http://www.ncbi.nlm.nih.gov/pubmed/25655405?dopt=Abstract

Alternate JournalExp. Parasitol.
Citekey25655405